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KMID : 0358920010280010072
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Journal of the Korean Academy of Pedodontics
2001 Volume.28 No. 1 p.72 ~ p.81
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HISTAMINE RELEASE INDUCED BY DENDROASPIS NATRIURETIC PEPTIDE FROM RAT PERITONEAL MAST CELLS
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Kim Jae-Gon
Hur Sun
Baik Byeong-Ju
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Abstract
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Dendroaspis natriuretic peptide (DNP), recently isolated from the venom of the green Mamba snake Dendroaspis angusticeps, is a 38-amino acid peptide containing a 17-amino acid disulfide ring structure similar to that of the natriuretic peptide family. The natriuretic peptide family was known to induce histamine release from human and rat mast cells, but there are no published data concerning the effects of DNP on histamine release from mast cells. The purpose of this study is to investigate whether DNP induces the histamine release from rat peritoneal mast cells (RMPCs) and to determine the mechanism of DNP-induced histamine release from RPMCs. After treatment of the various doses of DNP in RPMCs, the mast cell degranulation was observed with inverted microscopy and the histamine release was measured by radio-enzymatic assay. Calcium uptake and intracellular cyclic GMP level were measured by radioimmunoassays. DNP induced the mast cell degranulation. DNP released the histamine and increased the calcium uptake and the level of intracellular cyclic GMP of RPMCs, in a dose-dependent manner. The results indicate that DNP is capable of inducing histamine release from RPMCs by increasing of calcium uptake and intracellular cyclic GMP level.
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KEYWORD
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Dendroaspis natriuretic peptide (DNP), Mast cell, Histamine release
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